Shubha Khote studied at St. Teresa's High School, Charni Road and then graduated in English Literature from Wilson College. Her father Nandu Khote was a stage actor, and had also acted in the silent movies. Her aunt, Durga Khote, was also a well-known actress. Her uncle Nayampalli was also an actor. She is married to D. M. Balsavar, who was the Vice President of Marketing in Nocil. He appeared in a cameo in the Marathi movie Chimukla Pahuna (1968), which she produced and directed.

Her daughter, Bhavana Balsavar is also a TV actress.

She made her stage debut as child actor at age 4. Shubha Khote made her film debut in Seema (1955) as Putli. This role came to her since she was a good cyclist and her cycling attracted the 'Seema's team for her selection. Since then, she has starred in a large number of Hindi and Marathi movies, stage shows and TV serials. She was mostly starred opposite Mehmood and the pair became hit in Sasural, Bharosa, Ziddi, Chhoti Behan, Sanjh Aur Savera, Love in Tokyo, Grahasthi, Humrahi and Beti Bete. She also played negative roles in Paying Guest and Ek Duuje Ke Liye. In 1962, at the 9th Filmfare Awards, she received two nominations for Filmfare Award for Best Supporting Actress for Gharana and Sasural, though she lost to Nirupa Roy.^[5]

She has directed comedy plays such as Hera Pheri, Hum Dono, Bachelor's Wife and Let's Do it (2000).^[4][6] Her home production Bachelor's Wives (adapted from the Marathi play Gholat Ghol) had more than 40 shows in Mumbai and Aurangabad. Her TV show Zabaan Sambhalke (based on the Mind Your Language series) was a major hit.^[7]

Currently she is working in the Marathi teleserial Eka Lagnachi Teesri Goshta on Zee Marathi.

Movies

• Toilet Ek Prem Katha ( 2017 Film )
• Shararat (2002)
• Sirf Tum (1999)
• Koyla (1997)
• Waqt Hamara Hai (1993)
• Parda Hai Parda (1992)
• Saudagar (1991)
• Dil Hai Ki Manta Nahin (1991)
• Kishen Kanhaiya (1990)
• Khoon Bhari Maang (1988)
• Swarag Se Sunder (1986)
• Saagar (1985)
• Hum Dono (1985)
• Coolie (1983)
• Ek Duuje Ke Liye (1981)
• Naseeb (1981)
• Badalte Rishtey (1978)
• Gol Maal (1979)
• Benaam (1974)
• Mili (1975)
• Tumse Achha Kaun Hai (1969)
• Love in Tokyo (1966)
• Akashdeep (1965)
• Phoolon Ki Sej (1964)
• Ziddi (1964)
• Dil Ek Mandir (1963)
• Grahasti (1963)
• Sasural (1961)
• Anari (1959)
• Chhoti Bahen (1959)
• "Didi" (1959)
• Dekh Kabira Roya (1957)
• Paying Guest (1957)
• Seema (1955)

TV

• Ek Raja Ek Rani (1996)
• Jugni Chali Jalandhar
• Zabaan Sambhalke (1993)
• Andaaz (1998)
• Dam Dama Dam (1998-1999)
• Baa Bahoo Aur Baby (2010)
• Eka Lagnachi Teesri Goshta (2013 Marathi)

• Nominated – Filmfare Award for Best Supporting Actress – Gharana (1962)
• Nominated – Filmfare Award for Best Supporting Actress – Sasural (1962)