Rupali was born in Kolkata to a Hindu Bengali family. She is the daughter of acclaimed director Anil Ganguly, who made films like Kora Kagaz (1974) and Tapasya (1976). In one of the reunion interviews, Sumeet Raghavan disclosed that she is a Maharashtrian as well. She started acting when she was 7 years old with a role in the film Saaheb, following it up with an award winning performance in her father's film, Balidan. Her latest TV serials include the hilarious comedy serial on Star One Sarabhai vs Sarabhai in which she plays the character of Monisha.

She has participated in dance reality show Zara Nachke Dikha, and Bigg Boss (Season 1) (2006) and stunt/dare reality game show, Fear Factor – Khatron Ke Khiladi Level 2 (2009).^[6] She also gave voice over in animation film, Dashavatar (2008)^[7]

In 2000, she established an advertising agency in Mumbai.^[3]

Presently, she is a regular anchor of Bioscope — a films-based TV show telecast on national channel of Doordarshan. She also played in a New York Life commercial with Ravi Kishen.

Rupali got married to her longtime boyfriend, Ashwin K Verma on 6 February 2013. She gave birth to a baby boy on 25 August 2013.

She gave birth to her second son in 2015.

Fiction shows

Reality shows

• Satrangee Parachute (2011) - Sumitra C. Sharma
• Do Ankhen Barah Hath (1997) - Neeta
• Angaara (1997) - Gulabi