Rajsree first appeared as a child artiste in the Tamil film Naga Devadhai in 1956. She worked as child artiste in films like Bhaktha Ambarisha(1959) and Mangalyam(1960) in Telugu, Aasha Sundari(1960) in Kannada, Petraval Kanda Peruvazhvu (1960)and Baktha Sabari(1960) in Tamil. She debuted as the supporting young heroine with the Malayalam film Bharya (1962). Her character was named Gracy. With the success of her debut Malayalam film, Rajsree was always credited throughout her Malayalam film career as Gracy. Some of her famous Malaylam movies include Kadalamma (1963), Rebecca (1963), Pazhassi Raja (1964), Shakuntala (1965). However, she was cast only as supporting and second lead actress in Tamil films like Sengamala Theevu (1962), Nichaya Thaamboolam (1962), Thendral Veesum (1962), Kalai Arasi(1963), Yarukku Sondham (1963), Kattu Maina (1963) in the period 1962–63. Her portrayal as the courtesean Madhavi in the film Poompuhar brought her new recognition as supporting actress in 1964. It was the film Kadhalikka Neram Illai in 1964, which made her a household name as the main lead heroine. She started receiving more Tamil films from 1964 onwards.

However, she continued to be very selective as far as her choice of doing Malayalam movies was concerned. Her notable films in Malayalam as the lead heroine include Jail (1966), Kadamattathachan (1966), Anarkali (1966), Udhyogastha (1967), Karutha Rathrikal (1967), Kadal (1968), Dial 2244 (1968), Urangatha Sundary (1969), Sree Guruvayoorappan (1972 film), Sreemurukan, Devi Kanyaakumaari (1974). She did about 30 movies in Malayalam. Her last Malayalm film was Vishwaroopam (1978).

It was the Tamil comic classic, `Kaadhalikka Neramillai,' that catapulted Rajasree to national fame. She quoted in an interview "When I got a call from such a big banner as Chitralaya asking me to be present at the studio the next morning, I couldn't believe my ears. Many artistes had been considered for the role, I had heard. The song, `Anubavam Pudhumai,' was the sequence that was shot first. They liked it and I did the film,". Rajasree went on to play the same role in Telugu with Akkini Nageswara Rao and in Hindi with Shashi Kapoor — she was the only one to repeat the role in the remakes. She quoted in interview "I remember Nagesh pulling my leg often shouting out `Dhigilazhagi' whenever I was on the sets,". Hence both during the shooting of Bama Vijayam and everytime she met actor Nagesh, he would always call her as Dhiglazhagi as in the film, a thief refers to her by that name.

It was decided by Sridhar to remake the Kadhallikaa Neram Illai in Hindi as Pyar Kiye Jaa and he retained only Rajasree from the original. Her debut Hindi film was a major box office hit in 1966. This led to her receiving films in Hindi as well. However none of her 4 films in Hindi became a hit except for her debut Hindi film.

She made her heroine debut in Telugu films with Constable Kuthuru (1962) opposite Kanta Rao but previously she has been already introduced as a child artist in Nagula Chavithi (1956). She was cast as the heroine of N.T.Rama Rao in films like Aggi Barata(1966), Pidugu Ramudu(1966), Aggi Veerudu(1969), Chitti Chellelu (1969).Her pairing opposite actor Kanta Rao was box office success. The pair Rajasree-Kanta Rao worked in Pakkalo Ballem (1965), Bangaru Timmaraju (1964), Saptaswaralu (1969), Pratigyna Palana (1966), Vijaya Simha(1965), Aggidora (1967), Bhoolokamlo Yamolokam (1966), Devudichchina Bharta (1968), Veera Pooja (1968), Ranabheri (1968), Aggimeeda Guggilam (1968), Oke Kutumbham (1970), Merupu Veerudu (1970), Maa Manchi Akkayya (1970), Prema Jeevulu, Bhale Ettu Chivaraku Chittu (1970), Raite Raju (1970).They worked in another 10 movies where they were not paired opposite each other.She did about 76 films in Telugu. Her last film in Telugu was Andame Anandham (1977).

Her debut film in Kannada was Kantheredu Nodu (1961). The Kannada film Thejaswini, produced by M.Pandaribai, made Rajasree a popular in Kannada film circle. She played second lead supporting roles in Kannada films like Swarna Gowri, Rathna Manjari, Karuneye Kutumbada Kannu, Chandra Kumara. She regularly starred in mythological period films like Santha Thukaram (1963), Shivarathri Mahathme, Shivagange Mahathme with either Rajkumar (actor) or Udaykumar or both in the lead roles. After achieving success in Tamil with Kadhalikka Neram Illai, she decided to do only lead heroine roles even in Kannada from 1964. Her Kannada films as lead heroine include Chandavalliya Thota, Satya Harishchandra (1965 Kannada film), Bala Nagamma (1966 film), Bhale Basava, Mr. Rajkumar (1970), Jwala Mohini, CID 72, C.I.D. Rajanna. She was part of the cast in 14 films in Kannada with Rajkumar (actor) in the lead role. She did 30 films in Kannada with her last movie being Devadhasi (1978).

She went to do comedy films as the lead heroine in Tamil to capitalize on her comic abilities like Bama Vijayam(1967), Anubavi Raja Anubavi (1967), Anubavam Pudhumai (1967), Neeyum Naanum (1968), Delhi Mapillai (1968). These films have developed classic cult status over the years. She then went on to get the second lead heroine roles in films with M.G.Ramachandran in the lead like Thaikku Thalaimagan (1967), Kudiyirundha Koyil (1968), Adimai Penn (1969), Pattikaattu Ponnaiya (1973), Netru Indru Naalai (1974) and Naalai Namadhe (1975). Her other notable films in Tamil include Kallum Kaniyagum, suspense thriller Selva Magal (1967), Siritha Mugam (1968), Patham Pasali (1970), Poi Sollathae (1971), Pattondru Ketten (1971), Yanai Valartha Vanampadi Magan, Amudha (1975), Veeduvarai Uravu (1976). Her last film in Tamil was Iravu 12 Mani (1978). She did more than 50 films in Tamil.

When she received MGR award in 2004 she quoted: "I've done three films with the CM J.Jayalalitha, and I've also acted with her mother Sandhya as her daughter."

Rajasree hails from a village in Eluru, Andhra Pradesh. Her mother tongue is Telugu. She was born with a sister and her father was a railway station master. Rajasree was ten years old when she came to Chennai. Rajasree's husband died suddenly when her child was just four. Yet the actress has kept up her promise to him to this day! "Many ask me why I can't do television now? But I'm reluctant," she says. However, she has not severed ties with the film world. "I still have many friends from the industry. Saroja Devi calls up whenever she's in town. In 2004 Sharada, Vanisri, Jayapradha, Sachu, Padmini, Rajasulochana and I met over lunch. In fact it was Sachu who first called me up and told me about the award," she says. Rajasree spends her time in meditation and being a devotee of Sathya Sai Baba, people can often spot her at Sundaram, their Chennai centre. But today she is a picture of contentment, leading a relaxed life and taking care of her old sister. In 2012, she along-with her friends Saroja Devi, Kanchana, Jamuna, P.Susheela, Anjali Devi, Rajasulochana, Sowcar Janaki, Sechu, Cho Ramaswamy, Jamuna, Vijayakumari met Jayalalithaa at personal reseidence Veda Nilayam of Jayalalithaa.

Yesteryear actress Rajasree was honoured with the MGR Award in 2004.

In order of languages in which she acted the most to least number of films.

This list is incomplete; you can help by expanding it.

Telugu

Tamil

Kannada

Malayalam: Credited as Gracy

Hindi

1. Pyar Kiye Jaa (1966) (as Rajasree of south) .... Nirmala/Nimmi-(remake of Kadhalikka Neramillai)
2. Naseehat (1967)
3. Payal Ki Jhankar (1968)
4. Mujrim Kaun? (1968)