Mrudula was born on 8 June to Muralidharan Nair, who is working as an engineer in F.A.C.T Kalamassery, and Latha Menon in Ernakulam, Kerala. She completed her secondary education at Assisi Vidyaniketan Public School, Ernakulam. She has a bachelor's degree from St. Teresa's College, Ernakulam. She is currently pursuing her PG in Media studies at M.O.P. Vaishnav College for Women, Chennai. Her only brother Mithun Murali is also an actor cum model.

Mrudula started as a child anchor in the program Dial & See telecast in Jeevan TV along with her brother Midhun Murali as co-anchor. She has acted numerous ad-films in Tamil and Malayalam and also shot for a Tamil film, the Ilaiyaraaja musical Kangalum Kavipaduthey in 2007, which did not get a theatrical release. She stepped into the film industry with the Malayalam film Red Chillies. She received critical acclaim for her role as Shenbagavalli in her debut Tamil film Nagaraja Cholan MA, MLA.

She had no theatrical releases in 2014 but appeared in a short film titled Maniyara. Her upcoming Tamil film is Chikkikku Chikkikichu, a romantic comedy that unfolds during a train journey.^[5] She has been signed as one of the female leads in the Malayalam films Enthoru Bhagyam, which will feature her as Heera Samson, a modern girl from Bangalore, who is "bubbly and lively".^[6]

Mrudula Murali got her major break through with Bollywood movie Raagdesh where she essayed the role of Captain Lakshmi Sehgal. The movie and her role received praising from various corners. Her acting and dialogue mention received special mention in reviews. She at present is acting in Tamil movie Pistha starring Shirish Sharavanan of Metro movie fame and also in Ulagam Vilakku Varudhu directed by Thambi Ramaiah starring his son Umapathy.

• Dial & See telecast (Jeevan TV)
• The Happiness Project (Kappa TV) as Associate producer