Bopanna's family hails from Kodagu (also known by its Anglicised former name of Coorg). She was born on 4th December 1982. Daisy did her schooling from Aurobindo School, pre-university at Kumarans College and graduated with a Bachelor of Fine Arts degree from Chitrakala Parishat in Bangalore, Karnataka. She is married to Amit Jaju, a partner with the forensic practice of Ernst & Young and son of Prakash Jaju. Her brother is an eminent advertising professional working in Mumbai.

Being academically proficient and coming from an intellectual and highly educated family, Daisy was interested in pursuing a career in advertising as a copywriter. But the call of the entertainment industry proved too strong and she put on hold her intellectual ambitions and opted to work in front of the camera.

She started with theater, working for a short time with B. Jayasri’s Spandana theatre camp and also contemporary English theatre^[3] with Preetam Koilpillai, and never looked back. Her first movie Bimba (The Image) was sent to the prestigious Berlin & Frankfurt Film Festival, winning international awards. The movie, directed by Kavitha Lankesh, explored the exploitation of child artistes in the film industry. Her later work in Bhagavan with Darshan earned her the tag of "Spicy Daisy".^[5]

After that Daisy went on to act in many films such as Rama Shyama Bhama, Thavarina Siri, Aishwarya, Satyavaan Savitri, Gaalipata, Olave Jeevana Lekkachara, Crazy Loka and other films. Daisy proved that she is a good performer in all these films. Daisy event went on to act in Hindi films like Garam Masala where she was seen opposite Akshay Kumar and John Abraham. Though the film was a hit, Daisy had to wait for another two years to find herself a next project. The next project came in the form of United Six which was average at the box-office.^{[citation needed]}

She starred in more than 150 regional ads before being cast in the Priyadarshan movie Garam Masala. She has also starred in a Tamil and Telugu film, opposite leading stars like Kamal Hassan, Ravi Teja and Ganesh. Her first ad film was with veteran ad film maker Rajiv Menon.^{[citation needed]}