Suhasini was born in Patna where she spent the early part of her childhood. She lost her father when she was only three and was brought up by her mother, noted documentary filmmaker and film historian Vijaya Mulay. She was attracted toward films because of her.

In 1965 she was chosen by Pears Soap to be its model. It was this ad film which caught Mrinal Sen's attention and he signed her for Bhuvan Shome (1969).

Though Bhuvan Shome proved to be a milestone in Indian cinema, Suhasini did not pursue acting as a career. Instead she enrolled at the prestigious McGill University in Montreal, Canada for a course in agricultural technology with specialization in soil chemistry and microbiology. She also obtained a degree in mass communication, and majored in film, radio, TV, journalism and print from the same university.

Suhasini returned to India in 1975 and worked as an assistant to Satyajit Ray in the Bengali film Jana Aranya. Later she joined Mrinal Sen as an assistant director in Mrigaya. Since then, she has been actively producing films and has made over 60 documentaries. She achieved national awards for four of them.

Almost 30 years after Bhuvan Shome, she made a great comeback to mainstream Bollywood cinema, through Gulzar's Hu Tu Tu for which she received another national award as the best supporting actress. She has been playing mother roles in Bollywood since then. She has worked on Jaane Kya Baat Hui, a television serial. Currently she completed her latest film PEEDA directed by Hemant Verma. PEEDA recently received best feature film award at FOG film festival USA.

Suhasini Mulay is used to living alone. She was in a long live-in relationship that ended in 1990. On 16 January 2011 she got married at Arya Samaj to a physicist, Prof. Atul Gurtu.^[1][5]

• 2000: Won: Filmfare Best Supporting Actress Award - Hu Tu Tu

• Chaldi Da Naam Gaddi
• Maan (Serial)
• Jabb Love Hua
• Kulvaddhu
• Piya Ka Ghar
• Mamta
• Jaane Kya Baat Hui
• Tithir Atithi
• Ek Tha Rusty (Miss Bean)
• Virasaat
• Princess Dollie Aur Uska Magic Bag
• Geet Hui Sabse Payare (Star One) (Maan's grandmother)
• Dil Se Diya Vachan
• "Kya Mast Hai Life" (TV show on Disney Channel)
• Hum- Ek Chote Gaon Ki Badi Kahani (Retelecasting and remake of Hum Log)
• Devon Ke Dev...Mahadev (Parvati's grandmother)
• Hongey Judaa Na Hum (Aniruddh's grandmother)
• Desh Ki Beti Nandini (Rajveer's grandmother)
• Udaan (Shakuntala Singh)
• Everest (Shikha maa)
• Warrior High (Principal Anvesha Rajput)